Lung protection by inhalation of exogenous solubilized extracellular matrix
RatOtherSprague-Dawleyn = n = 4 per group for extracellular matrix or saline in each…Intratracheal instillation via a 14-gauge tracheal cannula, or nebulisation through the tracheal cannula using a vibrating mesh nebuliser generating 4 to 6 micrometre droplets3.2 mg/mL preparation, 1.5 mg delivered per animal; in vitr…Single dose given before injury, immediately before recovery from anaesthesia a…
Model: Hyperoxia-induced acute lung injury, with anaesthetised intubated rats exposed either to continuous 90% oxygen for 3 days or to intermittent 90% oxygen for 24 h alternating with 21% oxygen for 24 h over three cycles across 6 days; parallel in vitro model of human A549 lung epithelial cells exposed to 95% oxygen for 24 h
Compared with saline, extracellular matrix treatment significantly reduced hyperoxia-induced alveolar septal thickening, oedema and exudation, and lowered caspase-8 activity by 14 to 18% during both continuous and intermittent hyperoxia and protein carbonyl by about 8% in both. Oxidative DNA damage…
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