Astragalus polysaccharide restores autophagic flux and improves cardiomyocyte function in doxorubicin-induced cardiotoxicity.
MixedChemical/toxinC57BL/6, specific pathogen free; in vitro myocytes from 1 to 3 day old Sprague-Dawley ratsMalen = In vivo n = 8 for body weight, heart weight to tibia length…Oral gavage; doxorubicin intraperitoneal1.5 g/kg/day in vivo; 50 micrograms/mL in vitroPre-treatment for 3 days before doxorubicin injection
Model: Doxorubicin-induced cardiotoxicity and heart failure with a single intraperitoneal injection of doxorubicin 20 mg/kg and euthanasia 5 days later; in vitro doxorubicin 0.5 microM for 24 hours
Astragalus polysaccharide pre-treatment restored autophagic flux, improved ejection fraction and fractional shortening and reduced doxorubicin-induced apoptosis (TUNEL 22.28% with doxorubicin versus 11.53% with doxorubicin plus extract), with the benefit reversed by rapamycin.
Extraction: extract · Supplied extraction confidence: 0.9. Machine-extracted fields require source verification.