Electroporation-mediated delivery of the FER gene in the resolution of trauma-related fatal pneumonia.
MouseCrushOther / unspecifiedC57BL/6 wild type (Harlan)malen = 18 animals assessed for blood cultures across time points;…Intrapulmonary hypopharyngeal instillation with external chest electroporation150 micrograms of plasmid DNA suspended in 100 mM NaCl, del…Same day as injury, between lung contusion and bacterial inoculation, that is w…
Model: Unilateral closed-chest blunt lung contusion by cortical contusion impactor followed 6 h later by Klebsiella pneumoniae (strain 43816, serotype 2) pneumonia
FER gene delivery gave 90 per cent seven-day survival compared with 20 per cent for untreated lung contusion plus pneumonia and 0 per cent for the Na/K-ATPase group (p = 0.004 by log-rank). It increased bronchoalveolar monocytes, raised Nos-2, Fizz1 and IL-1 beta expression, reduced lung and blood…
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