Destabilization of Lysophosphatidic Acid Receptor 1 Reduces Cytokine Release and Protects Against Lung Injury.
MouseEndotoxin/LPSC57BL/6n = 6 to 8 mice per groupIntratrachealMitoxantrone 0.25 mg/kg body weight; lentiviral USP11 shRNA…Mitoxantrone given intratracheally prior to lipopolysaccharide challenge; lenti…
Model: Intratracheal lipopolysaccharide induced acute lung injury (2 mg/kg body weight for 24 h)
Both lentiviral knockdown of USP11 and intratracheal mitoxantrone attenuated lipopolysaccharide induced increases in bronchoalveolar lavage protein, IL-6 and KC, and reduced neutrophil influx into alveolar spaces, consistent with reduced LPA1 stability and diminished LPA1-CD14 signalling.
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