Microglial activation induced by the alarmin S100B is regulated by poly(ADP-ribose) polymerase-1.
MouseTBIUnspecifiedC57BL/6 wild-type and PARP-1 knockout (backcrossed more than 10 generations onto C57BL/6)MaleNot stated for in vivo veliparib; S100B injected intrastriatally by stereotaxic injector under isoflurane and nitrous oxide anaesthesia; cell culture studies used S100B at 200 ng/mL (about 10 nM) up to 2000 ng/mL, with 20 ng/mL IFN-gamma co-incubation for nitric oxide measurements
Model: Intrastriatal needle track injury and intrastriatal injection of the alarmin S100B (striatal coordinates 1.0 mm anterior, 1.9 mm lateral, 3.5 mm deep relative to bregma)
S100B induced microglial process retraction, soma enlargement, upregulation of IL-1beta, TNF-alpha and iNOS, and release of MMP9 (about 2-fold) and nitric oxide (about 10-fold). Most of these effects were attenuated in PARP-1 knockout microglia and by veliparib, although MMP9 and nitric oxide relea…
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