Pretreatment with bone marrow-derived mesenchymal stromal cell-conditioned media confers pulmonary ischemic tolerance.
RatIschaemia-reperfusionOther / unspecifiedLong-Evans (pathogen-free, Harlan Sprague-Dawley)Malen = n=10 per conditioned media clone group; n=15 media-only con…Intratracheal instillation200 microlitres of conditioned media per animal30 minutes before ischaemia (pretreatment)
Model: Left lung warm ischaemia-reperfusion: left hilar non-crushing clamp for 90 minutes followed by 4 hours of reventilation and reperfusion
Five of seven conditioned media clones reduced injury severity; clones O and H gave the largest reduction in lung permeability (median 0.086, p=0.0021 and p=0.028). Protective conditioned media significantly reduced BAL IL-1 beta and TNF alpha (both p<0.0001) and absolute neutrophil counts (p=0.000…
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