The Development of Macrophage-Mediated Cell Therapy to Improve Skeletal Muscle Function after Injury.
MouseIschaemia-reperfusionLimb / skeletal muscleC57BL/6Male and femalen = Control 17, saline 7, M0 5, M1 6, with 3 to 5 per group for…Intramuscular into the gastrocnemius2 x 10^6 cells per muscle in 60 to 100 microl PBS24 h after reperfusion
Model: Tourniquet induced hindlimb ischaemia and reperfusion, pneumatic tourniquet at 250 mmHg for 2 h
M1 polarised macrophages given 24 h after tourniquet ischaemia and reperfusion improved specific tension by about 15 per cent and reduced fibrosis and myofibre damage, whereas unpolarised M0 macrophages impaired functional recovery.
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