ROS-Mediated NLRP3 Inflammasome Activity Is Essential for Burn-Induced Acute Lung Injury.
RatBurnScaldSprague-DawleyMalen = 42 rats: sham n = 6, burn n = 24 (6 at each of 6, 12, 24 an…Intraperitoneal in vivo; added to culture medium in vitro15 umol/kg in 1 mL/kg DMSO in vivo; 10 umol/L BAY11-7082 or…Immediately after burn injury
Model: 30% total body surface area full-thickness scald burn by immersion of the clipped dorsum in 92 C water for 18 s, with intraperitoneal saline resuscitation, producing burn-induced acute lung injury
Burn injury raised lung NLRP3 and Caspase-1 expression and IL-1beta and IL-18 from 12 h, peaking at 24 to 48 h. BAY11-7082 given immediately after the burn reduced NLRP3 and Caspase-1 expression, lung histopathological injury, BALF protein, myeloperoxidase activity and cytokine levels, and N-acetyl…
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