Acute Putrescine Supplementation with Schwann Cell Implantation Improves Sensory and Serotonergic Axon Growth and Functional Recovery in Spinal Cord Injured Rats.
RatSpinal cord injuryContusionFischer (F344)femalen = 34 rats in total: Schwann cells plus vehicle n = 11, plus a…putrescine by subcutaneous Alzet 2001 osmotic minipump; Schwann cells by intraspinal injection into the contusion epicentre at 1 mm depthputrescine 100 mM infused at 0.5 microlitres/h; Schwann cel…putrescine started either within 30 min of injury or 1 week after injury; Schwa…
Model: Moderate T9 contusion spinal cord injury using the MASCIS weight drop device, 10 g rod from 12.5 mm, after T8 laminectomy
Adding putrescine to Schwann cell implants increased implant size with delayed delivery, increased serotonergic and CGRP axon growth and improved BBB score and subscore for both timings, with reduced grid walk footfalls only after acute delivery.
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