Nanoparticle facilitated inhalational delivery of erythropoietin receptor cDNA protects against hyperoxic lung injury.
RatOtherSprague-Dawleyn = 4 animals per group in the hyperoxia experimentInhalation of nebulised aerosol (4 to 6 micrometre droplets) delivered through a tracheal cannula over 3 min under ketamine 50 mg/kg and xylazine 5 mg/kg intraperitoneal anaesthesia2 mg nebulised nanoparticles per animal; cDNA loading 10% w…Single administration 7 days before hyperoxic exposure
Model: Hyperoxia, 90% inspired oxygen in an environmental chamber for 3 days
A single nebulised dose of EpoR cDNA nanoparticles raised pulmonary EpoR expression and ERK1/2 and STAT5 phosphorylation for up to 21 days and reduced hyperoxia-induced septal thickening, oedema, caspase-8 activity and oxidative damage to DNA, protein and lipid, with no effect on the hyperoxia-indu…
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