Histamine deficiency exacerbates myocardial injury in acute myocardial infarction through impaired macrophage infiltration and increased cardiomyocyte apoptosis.
MouseOtherHDC-EGFP BAC transgenic and HDC knockout mice on Balb/C and C57BL/6 backgrounds; H9c2 rat cardiomyoblasts used in vitron = 7 mice in the combined pyrilamine plus cimetidine group; 14…Intraperitoneal in vivo; culture medium in vitroHistamine 4 mg/kg/day intraperitoneally; pyrilamine 10 mg/k…Dosing started 3 days before coronary ligation and continued until euthanasia
Model: Acute myocardial infarction induced by permanent ligation of the left anterior descending coronary artery under isoflurane anaesthesia, with sham thoracotomy controls
Histamine deficiency raised CK-MB, enlarged infarct size, lowered ejection fraction and increased cardiomyocyte apoptosis while reducing early macrophage infiltration, and exogenous histamine reversed these changes; combined pyrilamine plus cimetidine markedly reduced 7 day survival (1 of 7 mice su…
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