Therapeutic Efficacy of E-64-d, a Selective Calpain Inhibitor, in Experimental Acute Spinal Cord Injury.
RatSpinal cord injuryContusionSprague DawleyMalen = Five groups (sham, SCI, E-64-d, methylprednisolone, E-64-d…IntravenousE-64-d 1 mg/kg in 1.5% DMSO vehicle; methylprednisolone 165…Immediately after injury
Model: Acute contusive spinal cord injury at T10 by laminectomy followed by modified Allen's weight-drop method (5 g weight dropped from 8 cm onto a 0.3 cm impounder)
E-64-d given immediately after injury reduced calpain 1 mRNA and protein, limited 68-kD NFP and MAP1B degradation, lowered caspase-3 activity and cytosolic AIF, reduced NeuN-TUNEL-positive neuronal apoptosis, reduced GFAP staining and COX-2 activity, and improved inclined plane angle and BBB scores…
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