Neutrophil elastase mediates acute pathogenesis and is a determinant of long-term behavioral recovery after traumatic injury to the immature brain.
MouseTBIControlled cortical impactNeutrophil elastase knockout (B6.129X1) and wild-type littermates, plus C57Bl/6J for the drug armMale for the C57Bl/6J drug cohort; not stated for the knockout and wild-type littermate cohortn = 175 mice in total; group sizes ranged from n=4 to n=8 for t…Intraperitoneal10 mg/kg (0.5 mg/mL in sterile isotonic saline)First dose 2 hours after injury
Model: Controlled cortical impact at postnatal day 21, 3.0 mm convex tip, 4.5 m/s velocity, 1.73 mm depth, 150 ms sustained depression, via 5.0 mm craniotomy; sham craniotomy without impact
Neutrophil elastase deletion reduced oedema, hippocampal cell death and oxidative stress and improved long-term spatial memory and hyperactivity without altering tissue loss, whereas acute pharmacological inhibition reduced acute cell death and oxidative stress but gave no long-term behavioural or…
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