Toll-like receptor 4 enhancement of non-NMDA synaptic currents increases dentate excitability after brain injury.
MixedTBIFluid percussionWistar; C57Bl6 wild-type and TLR4 knockout mice for controlsMale rats; mouse sex not statedn = Western blot n=6 rats per group; field recordings 9 slices…Ex vivo bath incubation of acute hippocampal slicesLPS-RS reported as 50 ng/mL in methods and 50 micrograms/mL…Slices prepared 3 to 5 days or 7 to 9 days after injury, with drug applied afte…
Model: Moderate lateral fluid percussion injury at 2 to 2.2 atm delivered through a 3 mm left craniotomy with intact dura; sham animals attached to the device without pendulum drop
One week after fluid percussion injury TLR4 antagonism reduced dentate population spike amplitude and non-NMDA EPSC amplitude and charge in granule and mossy cells, while the same antagonist increased excitability in sham controls, indicating bidirectional TLR4 modulation with no effect on NMDA cur…
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