Tumor necrosis factor alpha has an early protective effect on retinal ganglion cells after optic nerve crush.
MouseCrushPeripheral-nerve crushC57BL/6J wild-type, Tnf-deficient, Bax-deficient and cis-NF-kappaB-EGFP reporter mice on a C57BL/6J backgroundn = At least three retinas per condition for quantitative PCR;…Intraocular (intravitreal) injection2 microlitres of 50 ng or 100 ng intraocularly (results als…5 days before optic nerve crush, or 7 days after crush; TNF alone experiments a…
Model: Optic nerve crush causing retinal ganglion cell death
Tnf-deficient mice lost more ganglion cell layer neurons than wild-type at 14 days (19.46% versus 11.61%) and 21 days (26.55% versus 20.32%), and a single 100 ng TNF injection 5 days before crush reduced retinal ganglion cell loss by almost 50% at 1 week. Injection 7 days after crush had no signifi…
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