Influence of intrathecal delivery of bone marrow-derived mesenchymal stem cells on spinal inflammation and pain hypersensitivity in a rat model of peripheral nerve injury.
RatOtherSprague-DawleyFemalen = 9 to 11 animals per group for behavioural testing; 3 to 4 s…Intrathecal via indwelling lumbar catheter1 million cells in 15 microlitres OptiMEM per injection (66…Days 2, 3 and 4 after partial sciatic nerve ligation
Model: Partial sciatic nerve ligation (one-third to one-half of the right sciatic nerve trunk tightly ligated with 6-0 suture above the peroneal/tibial bifurcation), performed 9 days after intrathecal catheterisation
Repetitive intrathecal mesenchymal stem cell delivery did not alter partial sciatic nerve ligation-induced mechanical allodynia or thermal hyperalgesia over 20 days. Iba1 immunostaining in the ipsilateral dorsal horn was increased by ligation and only non-significantly reduced by the cells (94 per…
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