Dissecting the complement pathway in hepatic injury and regeneration with a novel protective strategy.
MouseIschaemia-reperfusionHepaticC57BL/6; CD59a-deficient and C3aR/C5aR double-deficient mice on a C57BL/6 backgroundn = Group sizes ranged from n = 2 to n = 12 depending on the ex…Intraperitoneal0.15 mg per animal (molar equivalent of the CR2-Crry dose o…Given immediately after surgery, just before the skin was sutured closed (that…
Model: Warm hepatic ischaemia-reperfusion (hepatic artery and portal vein occluded with a microaneurysm bulldog clamp for 30 min, followed by 6 or 24 h reperfusion), 70% partial hepatectomy, combined 30 min total hepatic ischaemia with 70% partial hepatectomy, and 90% partial hepatectomy
CR2-CD59 did not affect in vivo generation of C3 and C5 activation products but was as effective as the C3 activation inhibitor CR2-Crry at ameliorating hepatic ischaemia-reperfusion injury, indicating the membrane attack complex is the principal mediator of that injury. Unlike C3 or C5 inhibition,…
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