Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2.
MouseWoundFVB/N-derived inducible Tcf3 transgenic, ICR, athymic, Stat3 conditional knockout and Lcn2 knockout linesFemale in several experiments; not stated for ICR micen = Group sizes 4 to 13, for example n=5 per group for Tcf3 ind…Topical application to the wound bed; doxycycline given in chowRecombinant lipocalin-2 200 microlitres at 1 microgram/ml t…Topical treatments applied from immediately after wounding; doxycycline inducti…
Model: Full-thickness excisional dorsal skin wounds made with a 4 mm biopsy punch and 1 cm2 full-thickness wounds, plus in vitro keratinocyte scratch wounds
Tcf3 overexpression and topical Tcf3- or Lcn2-conditioned media accelerated wound closure (p<0.05) and increased keratinocyte migration by about 60% in vitro, while lipocalin-2 blockade or knockout delayed repair and recombinant lipocalin-2 partially rescued healing in Stat3 conditional knockout sk…
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