Impaired neuropathic pain and preserved acute pain in rats overexpressing voltage-gated potassium channel subunit Kv1.2 in primary afferent neurons.
RatOtherSprague-DawleyMalen = No overall total stated; n=5 rats for double labelling immu…Microinjection into the left L5 dorsal root ganglion after laminectomy2 microlitre per dorsal root ganglion; viral titre not reco…Before injury: spinal nerve ligation performed 4 weeks after viral injection in…
Model: L5 spinal nerve ligation (L5 spinal nerve tightly ligated with 3-0 silk and transected just distal to the ligature) and, separately, sciatic nerve axotomy about 1 cm distal to the exit of the spinal nerve roots; an intradermal capsaicin model (2 microgram in 50 microlitre) was used to test acute pain
Dorsal root ganglion Kv1.2 overexpression attenuated mechanical, cold and thermal hypersensitivity from day 3 to day 14 after spinal nerve ligation and on days 21, 28 and 35 in the maintenance paradigm compared with AAV5-EGFP, while capsaicin evoked nocifensive behaviour, baseline thresholds and mo…
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