Keratinocyte growth factor gene delivery via mesenchymal stem cells protects against lipopolysaccharide-induced acute lung injury in mice.
MouseEndotoxin/LPSC57BL/6 (mesenchymal stem cell donors were 2-week-old male C57BL/6, cells used at passages 4 to 6 after lentiviral transduction at MOI 20)Malen = n=5 per group per assay set; survival study 40 mice randomi…Intravenous via the tail vein; lipopolysaccharide given intratracheally2 hours after lipopolysaccharide
Model: Intratracheal Escherichia coli O55:B5 lipopolysaccharide at 10 mg/kg under pentobarbital anaesthesia (90 mg/kg)
Keratinocyte growth factor engineered cells reduced the lung wet to dry ratio and lavage protein (P<0.01 versus saline), lowered neutrophils, myeloperoxidase, TNF-alpha and IL-1 beta, raised IL-10, and reduced the 72 hour lung injury score to 7.9 plus or minus 2.14 versus 12.73 plus or minus 2.42 f…
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