Electrical stimulation of embryonic neurons for 1 hour improves axon regeneration and the number of reinnervated muscles that function.
RatOtherFischer (F344, inbred)FemaleIntraneural injection into the tibial nerve 10 to 15 mm from its entry into the gastrocnemii; stimulation via paired silver electrodes at the transplant site200,000 ventral spinal cord cells in 5 microL medium per an…Cells transplanted 1 week after denervation; stimulation applied immediately af…
Model: Peripheral denervation by left sciatic nerve transection 15 to 20 mm proximal to the tibial nerve entry into the gastrocnemii, with the proximal stump tied to adductor femoris to prevent spinal motoneuron reinnervation
One hour of stimulation, particularly at 1 Hz, increased total tibial nerve myelinated axons (727 +/- 109 vs 415 +/- 122 with no stimulation) and medial gastrocnemius myelinated axons (77 +/- 23 vs 51 +/- 19, p < 0.05), and raised the proportion of functionally reinnervated muscles to 77 to 87% ver…
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