Local arginase 1 activity is required for cutaneous wound healing.
MouseWoundC57BL/6, Arg1 conditional allele crossed to Tie2-Cre (with littermate controls), plus ovariectomised and 18-month-old aged miceFemaleLocal injection into the wound (vehicle phosphate-buffered saline)nor-NOHA 20 micrograms per 50 microlitresInjected at day minus 1 and day 0 with respect to wounding
Model: Two equidistant 1 cm full-thickness incisional or 6 mm excisional skin wounds healing by secondary intention
Arginase activity peaked at 5 days post wounding. Pharmacological inhibition with nor-NOHA and Tie2-cre-mediated Arg1 deletion each delayed healing, with increased wound area, reduced re-epithelialisation, increased and prolonged macrophage and neutrophil influx, more iNOS positive cells, reduced c…
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