Prevention of radiation-induced salivary gland dysfunction utilizing a CDK inhibitor in a mouse model.
MouseRadiationFVBFemalen = Flow cytometry at least 9 mice per treatment; real-time RT-…Intraperitoneal injection25 mg/kg or 100 mg/kgPretreatment, 2 hours before irradiation
Model: Targeted head and neck irradiation of the salivary glands under ketamine and xylazine anaesthesia with the body shielded by lead, single 5 Gy dose from a cobalt therapeutic irradiator (Theratron-80); a separate arm used fractionated 2 Gy per day for five consecutive days (cumulative 10 Gy)
Roscovitine before irradiation increased G2/M arrest and p21 expression, transiently reduced PCNA positivity and significantly reduced cleaved caspase-3 positive acinar cells at 24 and 48 hours. Stimulated salivary flow was preserved at day 3 with 100 mg/kg and at day 30 with both doses, including…
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