Oleic acid induces lung injury in mice through activation of the ERK pathway.
MouseChemical/toxinSwiss Webster, C57Bl/10 and C57Bl/10ScCr (TLR4 null)MaleIntraperitoneal, dissolved in DMSO and diluted in PBS10 mg/kg30 minutes before oleic acid administration
Model: Acute lung injury induced by intratracheal instillation of trisoleate (oleic acid), 1.25 micromol in 0.05 mL (approximately 10 mg/kg), under isoflurane anaesthesia; intratracheal lipopolysaccharide 500 ng in 0.05 mL used as a comparator injury
Pretreatment with U0126 significantly reduced neutrophil migration, oedema, lipid body formation and IL-6 production in oleic acid challenged mice, with IL-1 the exception. Increased inflammation after oleic acid was not dependent on TLR4, since TLR4-null mice were not protected.
Extraction: extract · Supplied extraction confidence: 0.8. Machine-extracted fields require source verification.