Prevention of hypoglycemia-induced neuronal death by minocycline.
Model: Insulin-induced severe acute hypoglycaemia under isoflurane anaesthesia and controlled ventilation: 10 U/kg regular insulin intraperitoneally, hypoglycaemia terminated after 30 min of isoelectric EEG by 0.2 mL of 50% glucose intravenously followed by continuous 1:1 50% glucose and Krebs-Henseleit infusion at 1.5 mL/h for 3 h; achieved blood glucose 0.40 plus or minus 0.03 mM (vehicle) and 0.38 plus or minus 0.01 mM (minocycline); mean arterial pressure maintained 120 to 180 mmHg; sham animals received the same insulin dose but were kept normoglycaemic with 25% glucose
Minocycline started 6 h after glucose reperfusion reduced hippocampal and cortical microglial activation and myeloperoxidase immunoreactivity, significantly increased surviving CA1 NeuN-positive neurons at 7 days and 8 weeks with fewer Fluoro-Jade B positive cells, and prevented hypoglycaemia-induc…
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