Alternatively-spliced extra domain A of fibronectin promotes acute inflammation and brain injury after cerebral ischemia in mice.
MouseIschaemia-reperfusionCerebral (MCAO/stroke)EDA+/+ and EDA+/- mice backcrossed more than 15 generations onto C57BL/6J; wild-type C57BL/6J controlsMaleIntravenous1 mg/kg30 min before the onset of ischaemia
Model: Focal cerebral ischaemia by 1 h middle cerebral artery occlusion with 23 h reperfusion
EDA-containing fibronectin worsened infarct size and inflammation, and the Toll-like receptor 4 inhibitor largely abrogated the increased brain injury and neurological deficits in EDA+/+ mice.
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