Concurrent transient activation of Wnt/beta-catenin pathway prevents radiation damage to salivary glands.
MouseRadiationKRT5-rtTA/tetO-Wnt1 inducible transgenic mice and BAT-gal/Axin2-lacZ Wnt reporter mice on C57BL/6 background; non-transgenic C57BL/6 controlsMale and female; efficacy assessed mainly in malesn = More than 5 animals per group in each experimentOral, doxycycline in chowDoxycycline chow 1 g/kg; radiation 15 Gy single doseConcurrent (doxycycline chow started 8 to 10 hours before irradiation), or 3 da…
Model: 15 Gy single dose irradiation to the head and neck region using a 6 MeV electron beam from a Clinac 2100C linear accelerator, under xylazine/ketamine anaesthesia
Concurrent transient Wnt1 induction prevented chronic salivary gland dysfunction after 15 Gy by suppressing apoptosis and preserving functional stem and progenitor cells, whereas induction 3 days before or after irradiation gave no significant benefit; activation was significant in submandibular gl…
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