Angiopoietin-1-expressing adipose stem cells genetically modified with baculovirus nanocomplex: investigation in rat heart with acute infarction.
MixedOtherImmunocompetent Lewis rats (Charles River)Female (rats); human cells from a male donorn = n = 8 per group for echocardiography and infarct size (24 r…Intramyocardial, three peri-infarct left ventricular injections using a 27 gauge needleApproximately 3 million hASCs in 300 microlitre culture med…15 minutes after coronary artery ligation
Model: Acute myocardial infarction by left thoracotomy through the fourth intercostal space and ligation of the left coronary artery 2 mm from its origin with 7-0 polypropylene suture, under 3% isoflurane
hASC-Ang1 gave higher cell retention, greater capillary density and reduced infarct size, with ejection fraction 46.28 plus or minus 6.3 per cent versus 36.35 plus or minus 5.7 per cent for unmodified hASC at 28 days after infarction.
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