TLR9 agonist protects mice from radiation-induced gastrointestinal syndrome.
MouseRadiationC57BL/6 for radioprotection and survival; BALB/c for macrophage transplantation and CT26 tumour studiesmalen = n=15 per cohort for survival studies; n=3 per group for his…subcutaneous for the TLR9 agonist; intravenous for the macrophage transplantationTLR9 agonist 1.0 mg/kg; MyD88 homodimerisation inhibitory p…1 h before or 1 h after whole body irradiation; 1 h before whole abdominal irra…
Model: radiation-induced gastrointestinal syndrome from single fraction whole body caesium-137 irradiation at 8.4, 9.4 or 10.4 Gy (236 cGy/min), or 14 Gy whole abdominal irradiation at 72 cGy/min with shielding
TLR9 agonist given 1 h before irradiation improved survival at 8.4, 9.4 and 10.4 Gy, reduced crypt apoptosis, increased crypt proliferation and restored xylose absorption; given 1 h after irradiation it mitigated injury only at 8.4 Gy, and protection was abolished by MyD88 inhibition.
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