Commensal and probiotic bacteria influence intestinal barrier function and susceptibility to colitis in Nod1-/-; Nod2-/- mice.
MouseChemical/toxinNod1(-/-); Nod2(-/-) on C57BL/6 background, specific pathogen-free and gnotobiotic altered Schaedler flora; Nod1(+/-); Nod2(+/-) littermate controlsMale and femaleOral gavage10^9 colony-forming units of B. breve per gavageDaily for 14 days before colitis induction
Model: Acute experimental colitis induced by dextran sodium sulfate
Nod1(-/-); Nod2(-/-) mice had increased paracellular permeability, decreased E-cadherin and lower colonic RegIII-gamma than littermate controls with greater colitis susceptibility. Altered Schaedler flora colonisation or B. breve NCC2950 supplementation normalised RegIII-gamma expression and reduce…
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