Inhibition of heat shock protein (molecular weight 90 kDa) attenuates proinflammatory cytokines and prevents lipopolysaccharide-induced liver injury in mice.
MouseEndotoxin/LPSC57BL/6Female and maleIntraperitoneal2.5, 5 or 30 mg/kg body weightAdministered with LPS; animals sacrificed at 2 or 18 hours
Model: Lipopolysaccharide-induced acute liver injury: 0.5 mg/kg body weight LPS (E. coli 0111:B4) in 0.1 mL intraperitoneally, with saline controls
17-DMAG significantly reduced LPS-induced serum alanine aminotransferase at all three doses independent of dose. Hepatic and serum TNF-alpha fell at 2.5 and 5 mg/kg and interleukin-6 at 5 mg/kg. HSF1 DNA binding and hsp70 rose dose-dependently, CD14 mRNA and NF-kappaB DNA binding fell without chang…
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